Kitazato Vitrification and Thawing Media

Kitazato vitrification media are the most versatile option for cryopreservation in your laboratory. Increase your efficiency by using the same media for vitrification and warming of oocytes and embryos, in all their stages of development, from zygote stage to blastocyst.

Kitazato media’s composition is entirely synthetic. Among their components, the following are notable:

  • Hydroxypropyl cellulose (HPC) prevents the risk of contamination, increases the survival rate in hatched blastocysts and reduces mechanical stress during warming.

  • Trehalose functions as an osmotic agent in place of sucrose. It provides greater safety in the process, improving the protection of the cellular membranes.

  • DMSO in combination with ethylene glycol in the media assures less toxicity and the best outcomes after warming. 

  • The incorporation of gentamicin prolongs the sterility of the media, guaranteeing greater safety in handling the solutions. 

VT601 / VT601US – Vitrification Media

  • 1 x 1.5 ml Basic Solution vial (BS)

  • 1 x 1.5 ml Equilibration Solution vial (ES)

  • 2 x 1.5 ml Vitrification Solution vial (VS)

VT602 / VT602US – Thawing Media

  • 2 x 4 ml Thawing Solution vial (TS)

  • 1 x 4 ml Diluent Solution vial (DS)

  • 1 x 4 ml Washing Solution vial (WS)

Kitazato Ultra-Fast Vitrification and Warming Media

Kitazato Ultra-Fast Vitrification and Warming Media streamline cryopreservation processes. Backed by strong scientific evidence and multicenter validation,
Ultra-Fast Vitrification Solutions guarantee high and consistent clinical outcomes.

Kitazato Ultra-Fast Vitri

  • Reduced exposure protocol: Only 2 minutes per procedure to minimize osmotic and chemical stress on the oocyte.

  • Superior molecular preservation: Transcriptomic analysis shows 97% fewer gene expression changes compared to standard vitrification, maintaining cellular integrity closer to that of fresh oocytes.

  • Refined for oocyte competence: The protocol improves morphokinetic profiles, and reduces post-ICSI degeneration, achieving a 82% time optimization.

  • Optimized kit with 1 extra VS vial: Enables vitrification of up to 3× more oocytes.

  • VT601UF: 1 × 1.5 mL ES + 3 × 1.5 mL VS . 

Kitazato Ultra-Fast Warm

  • 1-minute warming: 1-step procedure with no dilution or washing, simplifying workflow and reducing variability.

  • Less manipulation, more control: Fewer steps minimize errors and improve lab efficiency. Up to 10x faster than traditional warming.

  • Clinically proven: Validated in more than 10.000 specimens worldwide, with pregnancy and live birth rates comparable to standard protocols.

  • VT602UF: Available in a ready-to-use kit designed for efficiency: 4x4mL TS vials

Kitazato Cryotop® System

Cryotop® (Open System & Closed System) is a vitrification device with the best proven results on the market, also the one with the best user experience. Developed and manufactured in Japan, the Cryotop® allows you to optimize your routine and results to the maximum.

Highest Cooling and Warming Rates on the Market

Cooling and warming rates are essential parameters for the success of vitrification and thawing processes. These parameters, especially the warming ones, have a huge impact on specimens survival rates.

The Cryotop® vitrification device ensures a very high cooling and warming rates resulting into better survival and live birth rates

Key Features

  • Wider sheet for an easy loading of the specimens on the straw

  • Thin strip to allow high temperature exposure to specimens during vitrification and thawing

  • Diamond tip for an easy insertion into the cover straw

  • Identification marks to define the side where specimens should be loaded for quick location of the cryopreserved samples during thawing

  • Allows up to 4 specimens to be loaded per device

In the Closed System (SC), the design is intended to prevent the specimen from having direct contact with LN2

Cryotop® Flash

Its design incorporates all of the benefits of the original Cryotop® with an external cover that hermetically seals the Cryotop® Flash tip with weight, to prevent floating in liquid nitrogen.

Cryotop® Scoop

The Cryotop® Scoop is your convenient alternative for blastocyst vitrification. It delivers exceptional cooling and warming rates – among the fastest in its category – and efficient design features that streamline workflows while maintaining specimen safety and consistency.

Benefits:

• Visual markings for accurate orientation and handling. 

• U-shaped tips enable precise loading in minimal volumes while protecting specimens. 

• Slim design maximizes storage efficiency.

• Available in 5 colors for quick and easy specimen identification.